Effect of Exposure to 900 MHz GSM Mobile Phone Radiofrequency Radiation on Estrogen Receptor Methylation Status in Colon Cells of Male Sprague Dawley Rats

Journal of Biomedical Physics and Engineering

卷:7

Mokarram P.¹  Sheikhi M²  Saeb S.³  Mortazavi S. M. J.⁴  Shokrpour N.⁵ 

[1] Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran;

[2] Department of Radiobiology, School of Paramedical Sciences, Shiraz, Iran;

[3] Department of clinical biochemistry, school of medicine, Shiraz University of Medical Sciences, Shiraz, Iran;

[4] Ionizing and Non-ionizing Radiation Protection Research Center (INIRPRC), Shiraz University of Medical Sciences, Shiraz, Iran;

[5] Professor, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran;

关键词: Radiofrequency (RF);    DNA Methylation;    Colon Cancer;    Mobile Phone;    Microwave;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background: Over the past several years, the rapidly increasing use of mobilephones has raised global concerns about the biological effects of exposure to radiofrequency(RF) radiation. Numerous studies have shown that exposure to electromagneticfields (EMFs) can be associated with effects on the nervous, endocrine, immune,cardiovascular, hematopoietic and ocular systems. In spite of genetic diversity, theonset and progression of cancer can be controlled by epigenetic mechanisms such asgene promoter methylation. There are extensive studies on the epigenetic changes ofthe tumor suppressor genes as well as the identification of methylation biomarkers incolorectal cancer. Some studies have revealed that genetic changes can be induced byexposure to RF radiation. However, whether or not RF radiation is capable of inducingepigenetic alteration has not been clarified yet. To date, no study has been conductedon the effect of radiation on epigenetic alterations in colorectal cancer (CRC). Severalstudies have also shown that methylation of estrogen receptor α (ERα), MYOD,MGMT, SFRP2 and P16 play an important role in CRC. It can be hypothesized thatRF exposure can be a reason for the high incidence of CRC in Iran. This study aimedto investigate whether epigenetic pattern of ERα is susceptible to RF radiation and ifRF radiation can induce radioadaptive response as epigenetic changes after receivingthe challenge dose (γ-ray).Material and Method: 40 male Sprague-Dawley rats were divided into 4 equalgroups (Group I: exposure to RF radiation of a GSM cell phone for 4 hours and sacrificedafter 24 hours; Group II: RF exposure for 4 hours, exposure to Co-60 gammaradiation (3 Gy) after 24 hours and sacrificed after 72 hrs; Group III: only 3Gy gammaradiation; Group 4: control group). DNA from colon tissues was extracted to evaluatethe methylation status by methylation specific PCR.Results: Our finding showed that exposure to GSM cell phone RF radiation was capableof altering the pattern of ERα gene methylation compared to that of non-exposedcontrols. Furthermore, no adaptive response phenomenon was induced in the patternof ERα gene methylation after exposure to the challenging dose of Co-60 γ-rays.Conclusion: It can be concluded that exposure to RF radiation emitted by GSMmobile phones can lead to epigenetic detrimental changes in ERα promoter methylationpattern.

【 授权许可】

Unknown