Multiple Factors Determine the Structure of Bacterial Communities Associated With Aedes albopictus Under Artificial Rearing Conditions

Frontiers in Microbiology

卷:11

Shi Chen²  Kostas Bourtzis³  Naima Bel Mokhtar³  Hamidou Maiga⁴  Dongjing Zhang⁴  Vangelis Doudoumis⁴  George Tsiamis⁵  Antonios Augustinos⁶ 

[1] Forestry University, Fuzhou, China;

[2] Beneficial Insects Institute, Fujian Agriculture &

[3] Department of Environmental Engineering, University of Patras, Agrinio, Greece;

[4] Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria;

[5] Institut de Recherche en Sciences de la Santé/Direction Régionale de l’Ouest, Bobo-Dioulasso, Burkina Faso;

[6] Key Laboratory of Tropical Disease Control of the Ministry of Education, Sun Yat-sen University–Michigan State University Joint Center of Vector Control for Tropical Diseases, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China;

关键词: Aedes albopictus;    gut;    microbiota;    artificial rearing;    SIT;    culture-dependent;   

DOI  :  10.3389/fmicb.2020.00605

来源: DOAJ

【 摘 要 】

Insect symbionts are major manipulators of host’s behavior. Their effect on parameters such as fecundity, male mating competitiveness, and biological quality in general, can have a major influence on the effectiveness of the sterile insect technique (SIT). SIT is currently being developed and applied against human disease vectors, including Ae. albopictus, as an environment-friendly method of population suppression, therefore there is a renewed interest on both the characterization of gut microbiota and their exploitation in artificial rearing. In the present study, bacterial communities of eggs, larvae, and adults (both males and females) of artificially reared Ae. albopictus, were characterized using both culture-dependent and culture-independent approaches. Mosquito-associated bacteria corresponding to thirteen and five bacteria genera were isolated from the larval food and the sugar solution (adult food), respectively. The symbiont community of the females was affected by the provision of a blood meal. Pseudomonas and Enterobacter were either introduced or enhanced with the blood meal, whereas Serratia were relatively stable during the adult stage of females. Maintenance of these taxa in female guts is probably related with blood digestion. Gut-associated microbiota of males and females were different, starting early after emergence and continuing in older stages. Our results indicate that eggs contained bacteria from more than fifteen genera including Bacillus, Chryseobacterium, and Escherichia–Shigella, which were also main components of gut microbiota of female adults before and after blood feeding, indicating potential transmission among generations. Our results provided a thorough study of the egg- and gut-associated bacteria of artificially reared Ae. albopictus, which can be important for further studies using probiotic bacteria to improve the effectiveness of mosquito artificial rearing and SIT applications.

【 授权许可】

Unknown