Vancomycin-resistant Staphylococcus aureus isolated from camel meat and slaughterhouse workers in Egypt

Antimicrobial Resistance and Infection Control

卷:8

Heba El-Mahallawy¹  Mohamed El Hariri²  Gihan El-Moghazy³  Alaa Elsayed³  Khaled Al-Amery⁴  Rehab Elhelw⁴  Mahmoud Elhariri⁴  Dalia Hamza⁵ 

[1] Department of Animal Hygiene, Zoonoses and Animal Behaviour and Management, Faculty of Veterinary Medicine, Suez Canal University;

[2] Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Alazhar University;

[3] Department of Food Safety and Biotechnology, Regional Center for Food and Feed, Agricultural Research Center;

[4] Department of Microbiology, Faculty of Veterinary Medicine, Cairo University;

[5] Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University;

关键词: Dromedary camels;    Human;    S. aureus;    VRSA;    Abattoir;    Egypt;   

DOI  :  10.1186/s13756-019-0585-4

来源: DOAJ

【 摘 要 】

Abstract Background The emergence of vancomycin-resistant Staphylococcus aureus (VRSA) represents a challenge for the treatment of staphylococcal infections in both human and animals worldwide. Although VRSA has been detected in several animal species worldwide, data on the bacterial prevalence in dromedary camels and workers in camel slaughterhouses are scarce. Methods We investigated meat samples from 200 dromedary camel carcasses from three different abattoirs that were being prepared to be sent to the markets. Twenty hand swabs were voluntarily collected from the workers in the same abattoirs. Isolation and identification of the bacterial specimens from the samples were performed using conventional cultural techniques and biochemical identification and were confirmed by PCR amplification of the nuc gene. Antimicrobial susceptibility against nine antimicrobial agents commonly used in human and camels was tested using the disc diffusion method, and genetic analysis was performed by evaluating the mecA gene in phenotypically oxacillin (OXA)- and cefoxitin (FOX)-resistant isolates. The resistance of S. aureus to vancomycin (VAN) was tested by broth microdilution and confirmed by PCR targeting the vanA and vanB genes. The vanA and vanB genes were sequenced. Result S. aureus was detected in both camel meat (29/200, 14.5%) and in abattoir workers (11/20, 55%). Of the collected samples, 27% (8/29, camel) and 54% (6/11, human) were identified as VRSA. All VRSA isolates carried both the vanA and vanB genes. Additionally, all VRSA isolates were also classified as methicillin-resistant S. aureus (MRSA). The vanA amplicons of the isolates from human and camel meat were homologous and clustered with a Chinese reference isolate sequence. Conclusion This study demonstrated that VRSA is present in camel abattoirs in Egypt. Zoonotic transmission between animals and human is probable and reflects both a public health threat and a food safety concern.

【 授权许可】

Unknown