Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant

Marine Drugs

卷:15

Bon-Won Koo¹  Hak Jun Kim¹  Young Baek Hur²  Jun Hyuck Lee³  Chang Woo Lee³  Sun-Ha Park³ 

[1] Department of Chemistry, Pukyong National University, Busan 48513, Korea;

[2] Tidal Flat Research Institute, National Fisheries Research and Development Institute, Gunsan, Jeonbuk 54014, Korea;

[3] Unit of Polar Genomics, Korea Polar Research Institute, Incheon 21990, Korea;

关键词: antifreeze proteins;    ice-binding proteins;    ice recrystallization inhibition;    cryoprotectant;    slow-freezing;    vitrification;   

DOI  :  10.3390/md15020027

来源: DOAJ

【 摘 要 】

Antifreeze proteins (AFPs) are biological antifreezes with unique properties, including thermal hysteresis(TH),ice recrystallization inhibition(IRI),and interaction with membranes and/or membrane proteins. These properties have been utilized in the preservation of biological samples at low temperatures. Here, we review the structure and function of marine-derived AFPs, including moderately active fish AFPs and hyperactive polar AFPs. We also survey previous and current reports of cryopreservation using AFPs. Cryopreserved biological samples are relatively diverse ranging from diatoms and reproductive cells to embryos and organs. Cryopreserved biological samples mainly originate from mammals. Most cryopreservation trials using marine-derived AFPs have demonstrated that addition of AFPs can improve post-thaw viability regardless of freezing method (slow-freezing or vitrification), storage temperature, and types of biological sample type.

【 授权许可】

Unknown