Muscadine or amla extracts standardized to ellagic acid content ameliorate glucolipotoxicity associated β-cell dysfunction via inhibition of IL-1β and improved insulin secretion

Food Production, Processing and Nutrition

卷:2

Jack N. Losso¹  Millicent Yeboah-Awudzi¹  Srikanth Earpina¹  Kristina J. Cook¹  Karen McDonough²  Sita Aggarwal³ 

[1] Chronic Degenerative Disease Prevention Laboratory, School of Nutrition and Food Science, Louisiana State University AgCenter, Louisiana State University System;

[2] School of Animal Science, Louisiana State University AgCenter, Louisiana State University System;

[3] William Hansel Cancer Prevention, Pennington Biomedical Research Center, Louisiana State University System;

关键词: Muscadine;    Amla;    Ellagic acid;    NIT-1 pancreatic β-cells;    glucose;    Palmitic acid;    Glucolipotoxicity;   

DOI  :  10.1186/s43014-020-00023-z

来源: DOAJ

【 摘 要 】

Abstract Glucolipotocixity induces IL-1 β secretion which impairs pancreatic β-cell insulin secretion. Ellagic acid and urolithin A have strong anti-inflammatory effect on cells. Muscadine and amla are very good sources of ellagic acid. The present study examined the effect of ellagic acid, ellagic acid-rich muscadine or amla extract, or urolothin A on inflammation in β cells under glucolipotoxic conditions. Rat NIT-1 β cells were incubated in glucolipotoxic conditions (33.3 mM glucose, 250 μM palmitic acid or 33.3 mM glucose + 250 μM palmitic acid with or without ellagic acid, ellagic acid-rich muscadine or amla extracts standardized to its ellagic acid content, or urolithin A). Inflammatory status was evidenced by ELISA analysis of insulin and IL-1β secretion. Ellagic acid-rich muscadine or amla extracts dose-dependently stimulated insulin secretion and down-regulated IL-1β better than pure ellagic acid, or urolithin A. Urolithin A did not statistically stimulate insulin secretion and did not inhibit IL-1β.

【 授权许可】

Unknown