Collecting wood core samples from Macassar ebony (Diospyros celebica Bakh.) for multi-purpose analysis using pickering punch

MethodsX

卷:9

Fifi Gus Dwiyanti¹  Muhammad Majiidu²  Lina Karlinasari²  Essy Harnelly³  Iskandar Zulkarnaen Siregar³  Ratih Damayanti³  Mohamad Rafi⁴  Meaghan Parker-Forney⁵  Dewi Anggraini Septaningsih⁶ 

[1] Corresponding author.;

[2] Department of Silviculture, Faculty of Forestry and Environment, IPB University, Bogor, Indonesia;

[3] Advanced Research Laboratory, IPB University, Bogor, Indonesia;

[4] Department of Biology, Faculty of Mathematics and Natural Science, Syiah Kuala University, Banda Aceh, Indonesia;

[5] Department of Forest Product, Faculty of Forestry and Environment, IPB University, Bogor, Indonesia;

[6] Research Center for Biomass and Bioproducts, National Research and Innovation Agency (BRIN), Cibinong, Bogor, Indonesia;

关键词: Technology-based sampling for multi-analyses;   

DOI  :  

来源: DOAJ

【 摘 要 】

Sample collection activities for a study of population genetics across the natural distribution of targeted tree species require a lot of resources, mainly if repeated field visits are necessary. Conventionally, population genetic studies use good sample material like leaves. In addition, cambium or small pieces of fresh wood can be used to replace leaf samples. Currently, restrictions from the permit regulation have caused only a limited number of samples that can be collected. Therefore, efficient use of samples must be designed to maximize their uses for research. Due to the small amount of successfully sampled materials, hence there are limitations to extend their uses for other analyses and are often sufficient only for genetic analysis. Therefore, innovation in sampling methods using pickering punch (https://www.agroisolab.com/pickering-punch) to collect ebony wood cores in this study is required to cover multi-analyses not only limited to genetics but also for other analyses such as isotopes, near-infrared spectroscopy (NIRs), anatomy, and chemical compounds. • Pickering punch is recommended for efficient wood core sample collection from ebony standing trees. • 323 wood core samples were successfully collected from 16 natural populations across Celebes (Sulawesi). • Multi-analyses studies on sampled wood cores are possible for ebony wood identification (e.g., species and origin/provenance).

【 授权许可】

Unknown