The effects of Intracerebellumal Injection of Estrogen on Anxiety-like Behavior and Parameters of Antioxidant System following Ethidium Bromide Injection in rat

Journal of Fasa University of Medical Sciences

卷:5

ali reza ali hemmati¹  homeira hatami²  shiva khezri³  faranak nejati³ 

[1] Department of Anatomy and Histology, Faculty of Medicine, Tabriz University of Medical Science, Tabriz, Iran.;

[2] Department of Animal Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran;

[3] Department of Biology, Facultyof Science, Urmia University, Urmia, Iran.;

关键词: Ethidium Bromide;    Estrogen;    Anxiety;    Cerebellum;    Oxidative stress;    Rat;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background & Objectives: Demyelination by Ethidium bromideis one of the ways that is usually used for preparing experimental models ofMS. Treatment with estrogen protects oligodendrocyts from cell poisoning andstops lipidic peroxidation. The aim of this study is the evaluation of the effects of estrogen on anxiety likebehavior and stress oxidative parameters after inducing demyelination byethidium bromide to the cortex of cerebellum in rats. Materials & Methods: Induction of demyelination wascarried out in cerebellum cortex by direct injection of Ethidium Bromide (EB) (0.01%). One week after injection of EB, animalswere treated with two doses of estrogen (2µg/μl and 4µg/μl) for 3 days. After the treatment stage, characteristicsof anxiety were assessed by using Elevated plus Maze  ; moreover , the parameters of stressoxidative were evaluated. One-way Analysis of Variance (ANOVA) was performed.  Results: The results of thisstudy showed that Open Arm Entry percentage and open arm time (anxiety index) in rats withestrogen treatment was significant relative to Ethidium Bromide group(p<0.01); in addition , activity value of glutathione peroxidase andcatalase showed significant increase compared to Ethidium Bromide group.Conclusion: Microinjection of estrogen candecrease anxiety as well asoxidative stress.

【 授权许可】

Unknown