Whole blood profiling of leprosy type 1(reversal) reactions highlights prominence of innate immune response genes

BMC Infectious Diseases

卷:18

Marcelo Ribeiro-Alves¹  Milton Ozório Moraes¹  Thiago Gomes de Toledo-Pinto¹  Léa Cristina Castellucci²  Paulo Roberto Lima Machado²  Nadja de Lima Santana³  Jamile Leão Rêgo³ 

[1] Fundação Oswaldo Cruz-FIOCRUZ;

[2] Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais;

[3] Programa de Pós-graduação em Ciências da Saúde da Universidade Federal da Bahia;

关键词: Leprosy reactions;    gene expression;    profile;    Parkin;    Pro-inflammatory;    Type-I IFN;    OASL;   

DOI  :  10.1186/s12879-018-3348-6

来源: DOAJ

【 摘 要 】

Abstract Background The major factors contributing for nerve damage and permanent disabilities in leprosy are type 1 or reversal reactions (RR) and type 2 or erythema nodosum leprosum (ENL). Gene profiling of leprosy reactions have shown that different pathways are activated during the course of reactions, which is consistent with the exacerbated immune response exhibited by these patients. Methods We used qPCR to screen a panel of 90 genes related to the immune response in leprosy in RNA-derived peripheral leukocytes of patients with (N = 94) and without leprosy reactions (N = 57) in order to define expression signatures correlated to RR or ENL. Results Our results show that there is a marked signature for RR in the blood, comprising genes mostly related to the innate immune responses, including type I IFN components, autophagy, parkins and Toll like receptors. On the other hand, only Parkin was differentially expressed in the ENL group. Conclusions The data put together corroborates previous work that brings evidence that an acute uncontrolled exacerbated immune response designed to contain the spread of M. leprae antigens might be cause of RR pathogenesis. Identifying a blood profile useful to predict leprosy reactions prior to its development might help to reduce the morbidity associated to this disabling disease.

【 授权许可】

Unknown