Identification of an Intermediate Step in Foamy Virus Fusion

Viruses

卷:12

Aurélie Dupont¹  Ivo M. Glück¹  Dorothee Ponti¹  Florian Perrotton¹  Don C. Lamb¹  Nicole Stanke²  Stefanie Richter²  Dirk Lindemann²  Kristin Stirnnagel²  Sylvia Hütter² 

[1] Department of Chemistry, Ludwig Maximilians-Universität München, Butenandtstraße 5-13, 81377 München, Germany;

[2] Medical Faculty “Carl Gustav Carus”, Institute of Virology, Technische Universität Dresden, Fetscherstr. 74, 01307 Dresden, Germany;

关键词: foamy virus;    viral fusion;    retrovirus;    envelope glycoprotein;    capsid;    single virus tracking;   

DOI  :  10.3390/v12121472

来源: DOAJ

【 摘 要 】

Viral glycoprotein-mediated membrane fusion is an essential step for productive infection of host cells by enveloped viruses; however, due to its rarity and challenges in detection, little is known about the details of fusion events at the single particle level. Here, we have developed dual-color foamy viruses (FVs) composed of eGFP-tagged prototype FV (PFV) Gag and mCherry-tagged Env of either PFV or macaque simian FV (SFVmac) origin that have been optimized for detection of the fusion process. Using our recently developed tracking imaging correlation (TrIC) analysis, we were able to detect the fusion process for both PFV and SFVmac Env containing virions. PFV Env-mediated fusion was observed both at the plasma membrane as well as from endosomes, whereas SFVmac Env-mediated fusion was only observed from endosomes. PFV Env-mediated fusion was observed to happen more often and more rapidly than as for SFVmac Env. Strikingly, using the TrIC method, we detected a novel intermediate state where the envelope and capsids are still tethered but separated by up to 400 nm before final separation of Env and Gag occurred.

【 授权许可】

Unknown