Hypoxic Physiological Environments in a Gas-Regulated Microfluidic Device

Micromachines

卷:10

Tae-Joon Jeon¹  JinHyuk Woo²  Insu Lee²  SunMin Kim²  Min Lee³ 

[1] Department of Bioengineering, Inha University, Incheon 22212, Korea;

[2] Department of Mechanical Engineering, Inha University, Incheon 22212, Korea;

[3] Division of Advanced Prosthodontics, University of California at Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA;

关键词: hypoxic condition;    microfluidic system;    computational simulation;    oxygen detection;    oxygen scavenger;   

DOI  :  10.3390/mi10010016

来源: DOAJ

【 摘 要 】

Hypoxic environment is known as one of the critical factors in various physiological/pathological processes. It is imperative to recapitulate oxygen level in microscale for human physiology/pathology induced by hypoxia. Herein, we propose an oxygen-regulating system that can be applied to in vitro tissue models. We fabricated a microdevice with a gas-permeable membrane, allowing oxygen diffusion without direct contact to cells. We verified the formation of oxygen level less than 2% O2 concentration inside the device through computational simulation and experiments. H9c2 heart myoblasts were exposed to hypoxic condition in the device, and their cell viability were investigated. We anticipate that our system will be integrated with a platform to study hypoxia-induced human physiology and pathology as an efficient oxygen-regulating system.

【 授权许可】

Unknown