Time-Resolved Fluorescence in Photodynamic Therapy

Photonics

卷:1

Michael S. Patterson¹  Joseph E. Hayward¹  Shu-Chi Allison Yeh²  Qiyin Fang² 

[1] Department of Medical Physics and Applied Radiation Sciences, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada;

[2] School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada;

关键词: time-resolved fluorescence;    fluorescence lifetime;    photodynamic therapy (PDT);    photosensitizers;    dosimetry;   

DOI  :  10.3390/photonics1040530

来源: DOAJ

【 摘 要 】

Photodynamic therapy (PDT) has been used clinically for treating various diseases including malignant tumors. The main advantages of PDT over traditional cancer treatments are attributed to the localized effects of the photochemical reactions by selective illumination, which then generate reactive oxygen species and singlet oxygen molecules that lead to cell death. To date, over- or under-treatment still remains one of the major challenges in PDT due to the lack of robust real-time dose monitoring techniques.Time-resolved fluorescence (TRF) provides fluorescence lifetime profiles of the targeted fluorophores. It has been demonstrated that TRF offers supplementary information indrug-molecular interactions and cell responses compared to steady-state intensity acquisition. Moreover, fluorescence lifetime itself is independent of the light path; thus it overcomes the artifacts given by diffused light propagation and detection geometries. TRF in PDT is an emerging approach, and relevant studies to date are scattered. Therefore, this review mainly focuses on summarizing up-to-date TRF studies in PDT, and the effects of PDT dosimetric factors on the measured TRF parameters. From there, potential gaps for clinical translation are also discussed.

【 授权许可】

Unknown