In vitro anti-allergic activity of Moringa oleifera Lam. extracts and their isolated compounds

BMC Complementary and Alternative Medicine

卷:19

Malina Jasamai¹  Jamia Azdina Jamal¹  Khairana Husain¹  Nur Zahirah Abd Rani¹  Endang Kumolosasi¹  Kok Wai Lam¹ 

[1] Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia;

关键词: Moringa oleifera;    Anti-allergic;    Isolation;    RBL-2H3;    Histamine;    Beta-hexosaminidase;   

DOI  :  10.1186/s12906-019-2776-1

来源: DOAJ

【 摘 要 】

Abstract Background Moringa oleifera Lam. is a commonly used plant in herbal medicine and has various reported bioactivities such as antioxidant, antimicrobial, anticancer and antidiabetes. It is rich in nutrients and polyphenols. The plant also has been traditionally used for alleviating allergic conditions. This study was aimed to examine the anti-allergic activity of M. oleifera extracts and its isolated compounds. Method M. oleifera leaves, seeds and pods were extracted with 80% of ethanol. Individual compounds were isolated using a column chromatographic technique and elucidated based on the nuclear magnetic resonance (NMR) and electrospray ionisation mass spectrometry (ESIMS) spectral data. The anti-allergic activity of the extracts, isolated compounds and ketotifen fumarate as a positive control was evaluated using rat basophilic leukaemia (RBL-2H3) cells for early and late phases of allergic reactions. The early phase was determined based on the inhibition of beta-hexosaminidase and histamine release; while the late phase was based on the inhibition of interleukin (IL-4) and tumour necrosis factor (TNF-α) release. Results Two new compounds; ethyl-(E)–undec-6-enoate (1) and 3,5,6-trihydroxy-2-(2,3,4,5,6-pentahydroxyphenyl)-4H-chromen-4-one (2) together with six known compounds; quercetin (3), kaempferol (4), β-sitosterol-3-O-glucoside (5), oleic acid (6), glucomoringin (7), 2,3,4-trihydroxybenzaldehyde (8) and stigmasterol (9) were isolated from M. oleifera extracts. All extracts and the isolated compounds inhibited mast cell degranulation by inhibiting beta-hexosaminidase and histamine release, as well as the release of IL-4 and TNF-α at varying levels compared with ketotifen fumarate. Conclusion The study suggested that M. oleifera and its isolated compounds potentially have an anti-allergic activity by inhibiting both early and late phases of allergic reactions.

【 授权许可】

Unknown