Osteocalcin Mediates Biomineralization during Osteogenic Maturation in Human Mesenchymal Stromal Cells

International Journal of Molecular Sciences

卷:18

Yi-Jeng Huang¹  Hao-Hsiang Wu¹  Yu-Tzu Tsao²  Oscar K. Lee²  Yu-An Liu³  Yi-Shiuan Liu³ 

[1] Institute of Biophotonics, National Yang-Ming University, Taipei 11221, Taiwan;

[2] Institute of Clinical Medicine, National Yang-Ming University, Taipei 11221, Taiwan;

[3] Stem Cell Research Center, National Yang-Ming University, Taipei 11221, Taiwan;

关键词: mesenchymal stromal cells (MSCs);    osteocalcin;    osteogenic differentiation;    non-collagenous protein;    mineralization;    hydroxyapatite;    Raman spectroscopy;   

DOI  :  10.3390/ijms18010159

来源: DOAJ

【 摘 要 】

There is a growing interest in cell therapies using mesenchymal stromal cells (MSCs) for repairing bone defects. MSCs have the ability to differentiate into osteoprogenitors and osteoblasts as well as to form calcified bone matrix. However, the molecular mechanisms governing mineralization during osteogenic differentiation remain unclear. Non-collagenous proteins in the extracellular matrix are believed to control different aspects of the mineralization. Since osteocalcin is the most abundant non-collagenous bone matrix protein, the purpose of this study is to investigate the roles of osteocalcin in mineral species production during osteogenesis of MSCs. Using Raman spectroscopy, we found that the maturation of mineral species was affected by osteocalcin expression level. After osteocalcin was knocked down, the mineral species maturation was delayed and total hydroxyapatite was lower than the control group. In addition, the expression of osteogenic marker genes, including RUNX2, alkaline phosphatase, type I collagen, and osteonectin, was downregulated during osteogenic differentiation compared to the control group; whereas gene expression of osterix was upregulated after the knockdown. Together, osteocalcin plays an essential role for the maturation of mineral species and modulates osteogenic differentiation of MSCs. The results offer new insights into the enhancement of new bone formation, such as for the treatments of osteoporosis and fracture healing.

【 授权许可】

Unknown