Biomarker Discovery of Acute Coronary Syndrome Using Proteomic Approach

Molecules

卷:26

Jiyeong Lee¹  SangHyun Park²  Miji Shin³  Sora Mun³  Hee-Gyoo Kang³ 

[1] Department of Biomedical Laboratory Science, School of Medicine, Eulji University, Uijeongbu 11759, Korea;

[2] Department of Internal Medicine, School of Medicine, Eulji University, Daejeon 34824, Korea;

[3] Department of Senior Healthcare, Graduate School, Eulji University, Seongnam 13135, Korea;

关键词: acute coronary syndrome;    biomarker;    diagnosis;    proteomics;   

DOI  :  10.3390/molecules26041136

来源: DOAJ

【 摘 要 】

Acute coronary syndrome (ACS) is a condition in which the coronary artery supplying blood to the heart is infarcted via formation of a plaque and thrombus, resulting in abnormal blood supply and high mortality and morbidity. Therefore, the prompt and efficient diagnosis of ACS and the need for new ACS diagnostic biomarkers are important. In this study, we aimed to identify new ACS diagnostic biomarkers with high sensitivity and specificity using a proteomic approach. A discovery set with samples from 20 patients with ACS and 20 healthy controls was analyzed using mass spectrometry. Among the proteins identified, those showing a significant difference between each group were selected. Functional analysis of these proteins was conducted to confirm their association with functions in the diseased state. To determine ACS diagnostic biomarkers, standard peptides of the selected protein candidates from the discovery set were quantified, and these protein candidates were validated in a validation set consisting of the sera of 50 patients with ACS and 50 healthy controls. We showed that hemopexin, leucine-rich α-2-glycoprotein, and vitronectin levels were upregulated, whereas fibronectin level was downregulated, in patients with ACS. Thus, the use of these biomarkers may increase the accuracy of ACS diagnosis.

【 授权许可】

Unknown