Accelerated Solvent Extraction as a Green Tool for the Recovery of Polyphenols and Pigments from Wild Nettle Leaves

Processes

卷:8

Ivanka Žutić¹  Fırat Çınar²  Valentina Kruk³  Danijela Bursać Kovačević³  Ena Cegledi³  Sandra Pedisić³  Verica Dragović-Uzelac³  Maja Repajić³ 

[1] Faculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, 10000 Zagreb, Croatia;

[2] Faculty of Engineering, University of Mersin, Çiftlikköy Kampüsü, 33343 Yenişehir, Mersin, Turkey;

[3] Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia;

关键词: Urtica dioica L.;    nettle leaves;    accelerated solvent extraction;    polyphenols;    chlorophylls;    carotenoids;   

DOI  :  10.3390/pr8070803

来源: DOAJ

【 摘 要 】

This study aimed to investigate the performance of accelerated solvent extraction (ASE) as a green approach for the recovery of polyphenols and pigments from wild nettle leaves (NL). ASE was operated at different temperatures (20, 50, 80 and 110 °C), static times (5 and 10 min) and cycle numbers (1–4) using ethanol (96%) as an extraction solvent. In order to compare the efficiency of ASE, ultrasound assisted extraction (UAE) at 80 °C for 30 min was performed as a referent. Polyphenol and pigment analyses were carried out by HPLC and antioxidant capacity was assessed by ORAC. Seven polyphenols from subclasses of hydroxycinnamic acids and flavonoids, along with chlorophylls a and b and their derivatives and six carotenoids and their derivatives were identified and quantified. Chlorogenic acid was the most abundant polyphenol and chlorophyll a represented the dominant pigment. ASE conditions at 110 °C/10 min/3 or 4 cycles proved to be the optimal for achieving the highest yields of analyzed compounds. In comparison with UAE, ASE showed better performance in terms of yields and antioxidants recovery, hence delivering extract with 60% higher antioxidant capacity. Finally, the potential of NL as a functional ingredient from natural sources can be successfully accessed by ASE.

【 授权许可】

Unknown