Study on the Genetic Structure Based on Geographic Populations of the Endangered Tree Species: Liriodendron chinense

Forests

卷:12

Peng-Yan Zhou¹  Li-Xing Hui¹  Li-An Xu¹  Zhou-Xian Ni¹  Shu-Jing Huang¹  Fa-Xin Yu² 

[1] Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;

[2] The Key Laboratory of Horticultural Plant Genetic and Improvement of Jiangxi Province, Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang 330096, China;

关键词: conservation biology;    genetic diversity;    genetic structure;    Liriodendron chinense;    phylogeography;    species distribution pattern;   

DOI  :  10.3390/f12070917

来源: DOAJ

【 摘 要 】

Liriodendron chinense (Hemsley) Sargent is a Class II protected plant in China as natural populations are on the verge of extinction. There is still a lack of systematic research on the genetic resources of its geographic populations. In this study, we used 20 pairs of SSR markers with high polymorphism to analyze a total of 808 L. chinense samples from 22 regions, and 63 Liriodendron tulipifera Linn samples from 2 regions were used as a comparison group. The results revealed a total of 78 alleles in L. chinense, and the average expected heterozygosity (He) was 0.558, showing a low level of genetic diversity. The degree of differentiation of L. chinense was high, with the differentiation coefficient (Fst) as high as 0.302, which is related to the low gene flow (Nm = 0.578). Based on the genetic structure, principal coordinate analysis (PCoA) and phylogenetic analysis of 24 Liriodendron spp. populations, L. chinense and L. tulipifera had obvious differentiation, while the differentiation between L. chinense geographic populations was very large and irregular. Inbreeding appears within the geographic populations, and the level of genetic diversity is very low. In order to protect the genetic diversity of L. chinense, in addition to protecting the existing population as much as possible, artificial cultivation should introduce materials from multiple populations.

【 授权许可】

Unknown