Let-7a-5p, miR-100-5p, miR-101-3p, and miR-199a-3p Hyperexpression as Potential Predictive Biomarkers in Early Breast Cancer Patients

Journal of Personalized Medicine

卷:11

Chiara Autilio¹  Flavio Mignone²  Antonina Rinninella²  Damiano Arciuolo³  Antonino Mulè³  Giovanni Scambia³  Paola Fuso³  Donatella Guarino⁴  Armando Orlandi⁴  Nicola Nicolotti⁴  Mariantonietta Di Salvatore⁴  Ettore Capoluongo⁴  Gianluca Franceschini⁴  Brunella Di Stefano⁴  Concetta Santonocito⁴  Alessandra Cassano⁴  Carlo Barone⁴  Alejandro Martin Sanchez⁴  Matteo Ramundo⁴  Giampaolo Tortora⁴ 

[1] Department of Biochemistry and Molecular Biology, Faculty of Biology and Research Institute, Universidad Complutense, Av. Sèneca, 2, 28040 Madrid, Spain;

[2] Department of Science and Innovation Technology, University of Piemonte Orientale, V.le Teresa Michel 11, 15121 Alessandria, Italy;

[3] Department of Woman and Child Health and Public Health, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Largo A. Gemelli 8, 00168 Rome, Italy;

[4] Faculty of Medicine and Surgery, Università Cattolica Del Sacro Cuore, Largo F. Vito 8, 00168 Rome, Italy;

关键词: subtypes breast cancer;    miRNAs;    breast cancer treatment;    chemotherapy;    integrated therapies;    next-generation-sequencing;   

DOI  :  10.3390/jpm11080816

来源: DOAJ

【 摘 要 】

Background: The aim of this study is to identify miRNAs able to predict the outcomes in breast cancer patients after neoadjuvant chemotherapy (NAC). Patients and methods: We retrospectively analyzed 24 patients receiving NAC and not reaching pathologic complete response (pCR). miRNAs were analyzed using an Illumina Next-Generation-Sequencing (NGS) system. Results: Event-free survival (EFS) and overall survival (OS) were significantly higher in patients with up-regulation of let-7a-5p (EFS p = 0.006; OS p = 0.0001), mirR-100-5p (EFS s p = 0.01; OS p = 0.03), miR-101-3p (EFS p = 0.05; OS p = 0.01), and miR-199a-3p (EFS p = 0.02; OS p = 0.01) in post-NAC samples, independently from breast cancer subtypes. At multivariate analysis, only let-7a-5p was significantly associated with EFS (p = 0.009) and OS (p = 0.0008). Conclusion: Up-regulation of the above miRNAs could represent biomarkers in breast cancer.

【 授权许可】

Unknown