Fluorescence lifetime imaging of fluorescent proteins as an effective quantitative tool for noninvasive study of intracellular processes

Journal of Innovative Optical Health Sciences

卷:11

Artem Pliss¹  Junle Qu²  Svitlana M. Levchenko² 

[1] Institute for Lasers, Photonics and Biophotonics, University at Buffalo, State University of New York, Buffalo, NY 14260-3000, USA;

[2] Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong Province 518060, P. R. China;

关键词: Fluorescence lifetime imaging;    fluorescent proteins;    bioimaging;    intracellular processes;   

DOI  :  10.1142/S1793545817300099

来源: DOAJ

【 摘 要 】

Fluorescence lifetime imaging (FLIM) is an effective noninvasive bioanalytical tool based on measuring fluorescent lifetime of fluorophores. A growing number of FLIM studies utilizes genetically engineered fluorescent proteins targeted to specific subcellular structures to probe local molecular environment, which opens new directions in cell science. This paper highlights the unconventional applications of FLIM for studies of molecular processes in diverse organelles of live cultured cells.

【 授权许可】

Unknown