Fasting impairs type 2 helper T cell infiltration in the lung of an eosinophilic asthma mouse model

FEBS Open Bio

卷:11

Tomoya Hayashi¹  Ryoma Yokoyama²  Risako Onodera²  Taishi Higashi²  Fumika Nakagawa²  Joe Inoue²  Yusuke Suzuki²  Keiichi Motoyama² 

[1] Division of Vaccine Science Department of Microbiology and Immunology The Institute of Medical Science The University of Tokyo (IMSUT) Tokyo Japan;

[2] Faculty of Pharmacy Graduate School of Pharmaceutical Sciences Kumamoto University Kumamoto Japan;

关键词: eosinophils;    eosinophilic asthma;    fasting;    IL‐33;    lung infiltration;    Th2;   

DOI  :  10.1002/2211-5463.13268

来源: DOAJ

【 摘 要 】

Eosinophilic asthma is a form of bronchial asthma that is caused by the pulmonary infiltration of eosinophils and accounts for approximately half of the patients with severe asthma. Several cell types of the immune system in synergy with the epithelial cells of the lung provoke an inflammatory response in patients with asthma. Recently, the effect of fasting on immune cells and inflammation has attracted considerable attention. Therefore, we examined whether fasting may serve as novel preventive strategy in patients with asthma. In our study, we employed a previously established mouse model of eosinophilic asthma. C57BL/6 mice were inoculated intranasally with interleukin‐33 and ovalbumin (OVA) in order to induce eosinophil infiltration in the lung and subjected to a 48‐h long fasting period directly after or 7 days postinoculation. We used flow cytometry to characterise infiltrated immune cells in the lung and measured the quantity of inflammatory cytokines as well as antigen‐specific immunoglobins (Ig) by ELISA. Our results indicated that fasting lowered the number of eosinophilic pulmonary infiltrates in the eosinophilic asthma model mice. Furthermore, fasting suppressed anti‐OVA IgG1 production. Fasting suppressed Th2 cytokine production by impairing Th2 accumulation in the lung. The findings suggest that fasting may be a novel preventive strategy for eosinophilic asthma.

【 授权许可】

Unknown