Study on the persistence of Zika virus (ZIKV) in body fluids of patients with ZIKV infection in Brazil

BMC Infectious Diseases

卷:18

for the ZIKABRA Study Team¹  Guilherme Amaral Calvet²  Patrícia Brasil²  Rafael Freitas de Oliveira Franca³  Maeve Brito de Mello⁴  Silvana Pereira Giozza⁵  Adele Schwartz Benzaken⁵  Ana Maria Bispo de Filippis⁶  Patrícia Carvalho de Sequeira⁶  Ximena Pamela Diaz Bermudez⁷  Camila Helena Aguiar Bôtto-Menezes⁸  Marcia da Costa Castilho⁸  Marcus Vinicius Guimarães de Lacerda⁸  Kayvon Modjarrad⁹  Nathalie Jeanne Nicole Broutet¹⁰  Robyn Meurant¹⁰  Sihem Landoulsi¹⁰  Edna Oliveira Kara¹⁰  Philippe Gaillard¹⁰ 

[1] ;

[2] Acute Febrile Illnesses Laboratory, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation;

[3] Aggeu Magalhães Research Center, Oswaldo Cruz Foundation;

[4] Department of Communicable Diseases and Health Analysis, Pan American Health Organization/World Health Organization;

[5] Department of STI, AIDS and Viral Hepatitis, Secretary for Health Surveillance, Ministry of Health Brazil;

[6] Flavivirus Laboratory, Oswaldo Cruz Institute/Oswaldo Cruz Foundation;

[7] Public Health Department, University of Brasilia, Pan American Health Organization/World Health Organization;

[8] Tropical Medicine Foundation Doctor Heitor Vieira Dourado (FMT-HVD);

[9] Walter Reed Army Institute of Research;

[10] World Health Organization;

关键词: Arbovirus;    Flavivirus;    Zika virus;    Body fluids;    Persistence;    Rt-Pcr;   

DOI  :  10.1186/s12879-018-2965-4

来源: DOAJ

【 摘 要 】

Abstract Background Zika virus (ZIKV) has been identified in several body fluids of infected individuals. In most cases, it remained detected in blood from few days to 1 week after the onset of symptoms, and can persist longer in urine and in semen. ZIKV infection can have dramatic consequences such as microcephaly and Guillain-Barré syndrome. ZIKV sexual transmission has been documented. A better understanding of ZIKV presence and persistence across biologic compartments is needed to devise rational measures to prevent its transmission. Methods This observational cohort study will recruit non-pregnant participants aged 18 years and above with confirmed ZIKV infection [positive reverse transcriptase-polymerase chain reaction (RT-PCR) test in blood and/or urine]: symptomatic men and women in ZIKV infection acute phase, and their symptomatic or asymptomatic household/sexual infected contacts. Specimens of blood, urine, semen, vaginal secretion/menstrual blood, rectal swab, oral fluids, tears, sweat, urine and breast milk (if applicable) will be collected at pre-established intervals and tested for ZIKV RNA presence by RT-PCR, other co-infection (dengue, Chikungunya, HIV, hepatitis B and C, syphilis), antibody response (including immunoglobulins M and G), plaque reduction neutralization test (if simultaneously positive for ZIKV and dengue), and ZIKV culture and RNA sequencing. Data on socio-demographic characteristics and comorbidities will be collected in parallel. Participants will be followed up for 12 months. Discussion This prolonged longitudinal follow-up of ZIKV infected persons with regular biologic testing and data collection will offer a unique opportunity to investigate the presence and persistence of ZIKV in various biologic compartments, their clinical and immunological correlates as well as the possibility of ZIKV reactivation/reinfection over time. This valuable information will substantially contribute to the body of knowledge on ZIKV infection and serve as a base for the development of more effective recommendation on the prevention of ZIKV transmission. Trial registration NCT03106714 . Registration Date: April, 7, 2017

【 授权许可】

Unknown