Differential Function of Endogenous and Exogenous Abscisic Acid during Bacterial Pattern-Induced Production of Reactive Oxygen Species in Arabidopsis

International Journal of Molecular Sciences

卷:20

Linli Luan¹  Guangzhen Zhou¹  Yufeng Song¹  Chaozu He¹  Leitao Tan¹  Qiuping Liu²  Qingbei Weng² 

[1] Hainan Key Laboratory of Sustainable Utilization of Tropical Bioresources, Hainan University, Haikou 570228, China;

[2] School of Life Science, Guizhou Normal University, Guiyang 570228, China;

关键词: abscisic acid;    reactive oxygen species;    Arabidopsis;    Pseudomonas syringae;    callose deposition;    salicylic acid;   

DOI  :  10.3390/ijms20102544

来源: DOAJ

【 摘 要 】

Abscisic acid (ABA) plays important roles in positively or negatively regulating plant disease resistance to pathogens. Here, we reassess the role of endogenous and exogenous ABA by using: 35S::ABA2, a previously reported transgenic Arabidopsis line with increased endogenous ABA levels; aba2-1, a previously reported ABA2 mutant with reduced endogenous ABA levels; and exogenous application of ABA. We found that bacterial susceptibility promoted by exogenous ABA was suppressed in 35S::ABA2 plants. The 35S::ABA2 and aba2-1 plants displayed elevated and reduced levels, respectively, of bacterial flagellin peptide (flg22)-induced H₂O₂. Surprisingly, ABA pre-treatment reduced flg22-induced H₂O₂ generation. Exogenous, but not endogenous ABA, increased catalase activity. Loss of nicotinamide adenine dinucleotide phosphate oxidase genes, RBOHD and RBOHF, restored exogenous ABA-promoted bacterial susceptibility of 35S::ABA2 transgenic plants. In addition, endogenous and exogenous ABA had similar effects on callose deposition and salicylic acid (SA) signaling. These results reveal an underlying difference between endogenous and exogenous ABA in regulating plant defense responses. Given that some plant pathogens are able to synthesize ABA and affect endogenous ABA levels in plants, our results highlight the importance of reactive oxygen species in the dual function of ABA during plant-pathogen interactions.

【 授权许可】

Unknown