Effect of Hydrated Ionic Liquid on Photocycle and Dynamics of Photoactive Yellow Protein

Molecules

卷:26

Hiroto Mizutani¹  Kana Onodera¹  Masahide Terazima²  Yusuke Nakasone²  Suhyang Kim²  Miu Hatakenaka³  Utana Umezaki³  Yoshifumi Kimura³ 

[1] Department of Applied Chemistry, Graduate School of Science and Engineering, Doshisha University, Kyotanabe 610-0321, Japan;

[2] Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan;

[3] Department of Molecular Chemistry and Biochemistry, Faculty of Science and Engineering, Doshisha University, Kyotanabe 610-0321, Japan;

关键词: hydrated ionic liquid;    photoactive yellow protein;    photocycle;    transient grating spectroscopy;    conformational change;   

DOI  :  10.3390/molecules26154554

来源: DOAJ

【 摘 要 】

The mechanism by which proteins are solvated in hydrated ionic liquids remains an open question. Herein, the photoexcitation dynamics of photoactive yellow protein dissolved in hydrated choline dihydrogen phosphate (Hy[ch][dhp]) were studied by transient absorption and transient grating spectroscopy. The photocyclic reaction of the protein in Hy[ch][dhp] was similar to that observed in the buffer solution, as confirmed by transient absorption spectroscopy. However, the structural change of the protein during the photocycle in Hy[ch][dhp] was found to be different from that observed in the buffer solution. The known change in the diffusion coefficient of the protein was apparently suppressed in high concentrations of [ch][dhp], plausibly due to stabilization of the secondary structure.

【 授权许可】

Unknown