Identification of Volatile Sulfur Compounds Produced by Schizophyllum commune

Journal of Fungi

卷:7

Takahito Toyotome¹  Masahiko Takino²  Maki Yahiro³  Katsuhiko Kamei³  Masahiro Takaya⁴ 

[1] Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-1 Inada-cho Nishi, Obihiro, Hokkaido 080-8555, Japan;

[2] Japan Application Center, Life Sciences and Chemical Analysis, Agilent Technologies Japan, Ltd., 9-1 Takakura-cho, Hachioji, Tokyo 192-8510, Japan;

[3] Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, Chiba 260-8673, Japan;

[4] The Tokachi Foundation, Obihiro, Hokkaido 080-2462, Japan;

关键词: allergic bronchopulmonary mycosis;    microbial volatile organic compounds;    Schizophyllum commune;    volatile sulfur compounds;   

DOI  :  10.3390/jof7060465

来源: DOAJ

【 摘 要 】

Schizophyllum commune is a causative agent of allergic bronchopulmonary mycosis, allergic fungal rhinosinusitis, and basidiomycosis. Diagnosis of these diseases remains difficult because no commercially available tool exists to identify the pathogen. Unique volatile organic compounds produced by a pathogen might be useful for non-invasive diagnosis. Here, we explored microbial volatile organic compounds produced by S. commune. Volatile sulfur compounds, dimethyl disulfide (48 of 49 strains) and methyl ethyl disulfide (49 of 49 strains), diethyl disulfide (34 of 49 strains), dimethyl trisulfide (40 of 49 strains), and dimethyl tetrasulfide (32 of 49 strains) were detected from headspace air in S. commune cultured vials. Every S. commune strain produced at least one volatile sulfur compound analyzed in this study. Those volatile sulfur compounds were not detected from the cultures of Aspergillus spp. (A. fumigatus, A. flavus, A. niger, and A. terreus), which are other major causative agents of allergic bronchopulmonary mycosis. The last, we examined H₂S detection using lead acetate paper. Headspace air from S. commune rapidly turned the lead acetate paper black. These results suggest that those volatile sulfur compounds are potent targets for the diagnosis of S. commune and infectious diseases.

【 授权许可】

Unknown