Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in Colombia

Frontiers in Public Health

卷:6

Michael J. Conway¹  Heman Shakeri²  Natasha Duggan³  Paula Rozo-Lopez⁴  Berlin L. Londono-Renteria⁴  Majid Jaberi-Douraki⁵  Tonya M. Colpitts⁶ 

[1] Central Michigan University College of Medicine, Mount Pleasant, MI, United States;

[2] Department of Anatomy and Physiology, Institute of Computational Comparative Medicine, Kansas State University, Manhattan, KS, United States;

[3] Department of Cell Biology, University of Miami Miller School of Medicine, Miami, FL, United States;

[4] Department of Entomology, Kansas State University, Manhattan, KS, United States;

[5] Department of Mathematics, Institute of Computational Comparative Medicine, Kansas State University, Manhattan, KS, United States;

[6] National Emerging Infectious Diseases Laboratories (NEIDL), Department of Microbiology, Boston University School of Medicine, Boston, MA, United States;

关键词: Aedes saliva;    D7;    antibody levels;    biomarker;    dengue;   

DOI  :  10.3389/fpubh.2018.00111

来源: DOAJ

【 摘 要 】

BackgroundDengue is one of the most geographically significant mosquito-borne viral diseases transmitted by Aedes mosquitoes. During blood feeding, mosquitoes deposit salivary proteins that induce antibody responses. These can be related to the intensity of exposure to bites. Some mosquito salivary proteins, such as D7 proteins, are known as potent allergens. The antibody response to D7 proteins can be used as a marker to evaluate the risk of exposure and disease transmission and provide critical information for understanding the dynamics of vector–host interactions.MethodsThe study was conducted at the Los Patios Hospital, Cucuta, Norte de Santander, Colombia. A total of 63 participants were enrolled in the study. Participants were categorized into three disease status groups, age groups, and socioeconomic strata. The level of IgG antibodies against D7 Aedes proteins was determined by ELISA. We used a statistical approach to determine if there is an association between antibody levels and factors such as age, living conditions, and dengue virus (DENV) infection.ResultsWe found that IgG antibodies against D7 proteins were higher in non-DENV infected individuals in comparison to DENV-infected participants. Also, the age factor showed a significant positive correlation with IgG antibodies against D7 proteins, and the living conditions (socioeconomic stratification), in people aged 20 years or older, are a statistically significant factor in the variability of IgG antibodies against D7 proteins.ConclusionThis pilot study represents the first approximation to elucidate any correlation between the antibody response against mosquito D7 salivary proteins and its correlation with age, living conditions, and DENV infection in a dengue endemic area.

【 授权许可】

Unknown