Determination of Ochratoxin A (OTA), Ochratoxin B (OTB), T-2, and HT-2 Toxins in Wheat Grains, Wheat Flour, and Bread in Lebanon by LC-MS/MS

Toxins

卷:11

Zouhair Attieh¹  Amira Al Matari¹  Saada Dakroub¹  Osama Yamani¹  Jomana Elaridi² 

[1] Department of Laboratory Science and Technology, American University of Science and Technology, Achrafieh 16-6452, Lebanon;

[2] Department of Natural Sciences, School of Arts and Sciences, Lebanese American University, P.O. Box 13-5053, Beirut 1102-2801, Lebanon;

关键词: Ochratoxin A;    ochratoxin B;    T-2 toxin;    HT-2 toxin;    wheat;    flour;    bread;    Lebanon;   

DOI  :  10.3390/toxins11080471

来源: DOAJ

【 摘 要 】

Cereals are prone to fungal infection during growth, harvesting, transportation, and/or storage. As a result, cereals such as wheat grains and wheat-derived products may be contaminated with mycotoxins leading to acute and chronic health exposure. The current study investigated the presence of the mycotoxins: ochratoxin A (OTA), ochratoxin B (OTB), T-2, and HT-2 toxins in samples of wheat grains (n = 50), wheat flour (n = 50), and bread (n = 37) from the main mills in Lebanon using LC-MS/MS. Accuracy ranged from 98−100%, recoveries from 93−105%, and intraday and interday precision were 5−7% and 9−12%, respectively. The tested wheat grains, wheat flour, and bread samples did not contain detectable levels of T-2 and HT-2 toxins and OTB. Four wheat flour samples (8% of flour samples) showed positive OTA levels ranging from 0.6−3.4 μg·kg⁻¹ with an arithmetic mean of 1.9 ± 0.2 μg·kg⁻¹. Only one sample contained an OTA concentration greater than the limit set by the European Union (3 μg·kg⁻¹) for wheat-derived products. This study suggests that mycotoxin contamination of wheat grains, wheat flour, and bread in Lebanon is currently not a serious public health concern. However, surveillance strategies and monitoring programs must be routinely implemented to ensure minimal mycotoxin contamination of wheat-based products.

【 授权许可】

Unknown