Exogenous GA3 Application Enhances Xylem Development and Induces the Expression of Secondary Wall Biosynthesis Related Genes in Betula platyphylla

International Journal of Molecular Sciences

卷:16

Shan Gu¹  Huiyan Guo²  Ping Hu²  Chuanping Yang²  Chunrui Zhang²  Yucheng Wang²  Chao Wang²  Yanmin Wang²  Yuanyuan Jia²  Huizi Liu² 

[1] Department of Life Science and Technology, Mudanjiang Normal College, Mudanjiang 157012, China;

[2] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University,26 Hexing Road, Harbin 150040, China;

关键词: Betula platyphylla SUK;    GA3;    seed germination;    xylem development;    secondary wall biosynthesis related genes;   

DOI  :  10.3390/ijms160922960

来源: DOAJ

【 摘 要 】

Gibberellin (GA) is a key signal molecule inducing differentiation of tracheary elements, fibers, and xylogenesis. However the molecular mechanisms underlying the effect of GA on xylem elongation and secondary wall development in tree species remain to be determined. In this study, Betula platyphylla (birch) seeds were treated with 300 ppm GA3 and/or 300 ppm paclobutrazol (PAC), seed germination was recorded, and transverse sectionsof hypocotyls were stained with toluidine blue; the two-month-old seedlings were treated with 50 μM GA3 and/or 50 μM PAC, transverse sections of seedling stems were stained using phloroglucinol–HCl, and secondary wall biosynthesis related genes expression was analyzed by real-time quantitative PCR. Results indicated that germination percentage, energy and time of seeds, hypocotyl height and seedling fresh weight were enhanced by GA3, and reduced by PAC; the xylem development was wider in GA3-treated plants than in the control; the expression of NAC and MYB transcription factors, CESA, PAL, and GA oxidase was up-regulated during GA3 treatment, suggesting their role in GA3-induced xylem development in the birch. Our results suggest that GA3 induces the expression of secondary wall biosynthesis related genes to trigger xylogenesis in the birch plants.

【 授权许可】

Unknown