A Blind Smear From LJ Medium Revealing Pseudomonas aeruginosa Talus Osteomyelits Afer Seeking for Osteotuberculosis in a 13-Year-Old Teenager: A Case Report

Case Reports in Clinical Practice

卷:5

Nasim Eftekhari¹  Shayan Shahabinejad²  Reza Rezaeizadeh³ 

[1] Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.;

[2] Department of Microbiology,School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.;

[3] Private Clinic, Kerman, Iran.;

关键词: Talus;    Lowenstein Jensen (LJ;    Pseudomonas;    Osteomyelits;    Malachite green;   

DOI  :  10.18502/crcp.v5i4.5058

来源: DOAJ

【 摘 要 】

The Lowenstein-Jensen is an egg-based microbiology medium that was designed for selective isolation of Mycobacterium spp. The main inhibitory composition in this medium is a synthetic dye called malachite green. Mycobacterium spp. is resistant to the dye by de-colorization and oxidation of it. Some non-tuberculosis organisms may also overcome this inhibitory ingredient by similar enzymatic activity. In the final inspection of the bone TB culture of a teenage patient, the blind smear revealed the Pseudomonas talus osteomyelitis. According to the present and similar experiences, the preparation of 1-2 blind smears from TB culture- negative specimens taken from sterile sites of the body may unmask mycobacteria with the atypical colony and non-tuberculosis microorganisms.

【 授权许可】

Unknown