Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress

Brazilian Journal of Microbiology

卷:44

Marcos D. Pereira¹  Joaquim F.M. da Silva¹  Elis C.A. Eleutherio¹  Rafael A. de Sá¹  Raquel M. de Souza¹  Frederico A.V. de Castro¹ 

[1] Universidade Federal do Rio de Janeiro;

关键词: propolis;    antioxidant;    oxidative stress;    Saccharomyces cerevisiae;   

DOI  :  

来源: DOAJ

【 摘 要 】

Propolis is a natural product widely used for humans. Due to its complex composition, a number of applications (antimicrobial, antiinflammatory, anesthetic, cytostatic and antioxidant) have been attributed to this substance. Using Saccharomyces cerevisiae as a eukaryotic model we investigated the mechanisms underlying the antioxidant effect of propolis from Guarapari against oxidative stress. Submitting a wild type (BY4741) and antioxidant deficient strains (ctt1∆, sod1∆, gsh1∆, gtt1∆ and gtt2∆) either to 15 mM menadione or to 2 mM hydrogen peroxide during 60 min, we observed that all strains, except the mutant sod1∆, acquired tolerance when previously treated with 25 µg/mL of alcoholic propolis extract. Such a treatment reduced the levels of ROS generation and of lipid peroxidation, after oxidative stress. The increase in Cu/Zn-Sod activity by propolis suggests that the protection might be acting synergistically with Cu/Zn-Sod.

【 授权许可】

Unknown