The Cytotoxicity and Genotoxicity of Three Dental Universal Adhesives—An In Vitro Study

International Journal of Molecular Sciences

卷:21

Adam Wawrzynkiewicz¹  Ireneusz Majsterek¹  Wioletta Rozpedek-Kaminska¹  Grzegorz Galita¹  Barbara Lapinska²  Monika Lukomska-Szymanska²  Jerzy Sokolowski² 

[1] Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-419 Lodz, Poland;

[2] Department of General Dentistry, Medical University of Lodz, 90-419 Lodz, Poland;

关键词: dental materials;    dental universal adhesives;    cytotoxicity;    genotoxicity;    flow cytometry;   

DOI  :  10.3390/ijms21113950

来源: DOAJ

【 摘 要 】

Dental universal adhesives are considered an useful tool in modern dentistry as they can be used in different etching techniques, allow for simplified protocol and provide sufficient bond strength. However, there is still no consensus as to their toxicity towards pulp. Thus, the present study aimed to evaluate the cytotoxicity and genotoxicity of three universal adhesives: OptiBond Universal, Prime&Bond Universal and Adhese in an in vitro experimental model, monocyte/macrophage cell line SC (ATCC CRL-9855). The cytotoxicity was measured by means of XTT assay, whereas the genotoxicity (comet assay) was evaluated based on the percentage of DNA present in the comet tail. Furthermore, the ability of the adhesives to induce apoptosis was analyzed using flow cytometry (FC) with the FITC annexin V/propidium iodide (PI) double staining. The analysis of the cell cycle progression was performed with FC using PI staining. OptiBond Universal presented significant, while Prime&Bond Universal and Adhese Universal had minimal cytotoxicity and genotoxicity towards human SC cells. Moreover, only OptiBond Universal increased the level of apoptosis in SC cell line. None of the adhesives showed significant cell cycle arrest, as revealed by FC analysis. Due to substantial differences in toxicity in in vitro studies of dental adhesives, there is a great need for further research in order to establish more reliable test protocols allowing for standardized methodology.

【 授权许可】

Unknown