Comprehensive Genome-Wide Association Analysis Reveals the Genetic Basis of Root System Architecture in Soybean

Frontiers in Plant Science

卷:11

Davoud Torkamaneh¹  Waldiodio Seck³  François Belzile³ 

[1] Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada;

[2] Département de phytologie, Faculté des sciences de l’agriculture et de l’alimentation (FSAA), Université Laval, Quebec, QC, Canada;

[3] Institut de biologie intégrative et des systèmes (IBIS), Université Laval, Quebec, QC, Canada;

关键词: candidate gene;    genome-wide association;    phenotypic variation;    resilient varieties;    rhizoboxes;    root system architecture;   

DOI  :  10.3389/fpls.2020.590740

来源: DOAJ

【 摘 要 】

Increasing the understanding genetic basis of the variability in root system architecture (RSA) is essential to improve resource-use efficiency in agriculture systems and to develop climate-resilient crop cultivars. Roots being underground, their direct observation and detailed characterization are challenging. Here, were characterized twelve RSA-related traits in a panel of 137 early maturing soybean lines (Canadian soybean core collection) using rhizoboxes and two-dimensional imaging. Significant phenotypic variation (P < 0.001) was observed among these lines for different RSA-related traits. This panel was genotyped with 2.18 million genome-wide single-nucleotide polymorphisms (SNPs) using a combination of genotyping-by-sequencing and whole-genome sequencing. A total of 10 quantitative trait locus (QTL) regions were detected for root total length and primary root diameter through a comprehensive genome-wide association study. These QTL regions explained from 15 to 25% of the phenotypic variation and contained two putative candidate genes with homology to genes previously reported to play a role in RSA in other species. These genes can serve to accelerate future efforts aimed to dissect genetic architecture of RSA and breed more resilient varieties.

【 授权许可】

Unknown