The Chromosome-Scale Genome of Melon Dissects Genetic Architecture of Important Agronomic Traits

iScience

卷:23

Guancong Deng¹  Jenella Garraway²  Zhongyuan Hu²  Jinghua Yang³  Mingfang Zhang³  Yongchao Niu³  Jinmin Lian³  Jingquan Yu³ 

[1] Key Laboratory of Horticultural Plant Growth and Development, Ministry of Agriculture and Rural Affairs, Hangzhou 310058, China;

[2] Biozeron Shenzhen, Inc., Shenzhen 518081, China;

[3] Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, China;

关键词: Biological Sciences;    Plant Biology;    Genomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Comparative and evolutionary genomics analyses are the powerful tools to provide mechanistic insights into important agronomic traits. Here, we completed a chromosome-scale assembly of the “neglected” but vital melon subspecies Cucumis melo ssp. agrestis using single-molecule real-time sequencing, Hi-C, and an ultra-dense genetic map. Comparative genomics analyses identified two targeted genes, UDP-sugar pyrophosphorylase and α-galactosidase, that were selected during evolution for specific phloem transport of oligosaccharides in Cucurbitaceae. Association analysis of transcriptome and the DNA methylation patterns revealed the epigenetic regulation of sucrose accumulation in developing fruits. We constructed the melon recombinant inbred lines to uncover Alkaline/Neutral Invertase (CINV), Sucrose-Phosphatase 2 (SPP2), α-galactosidase, and β-galactosidase loci related to sucrose accumulation and an LRR receptor-like serine/threonine-protein kinase associated with gummy stem blight resistance. This study provides essential genomic resources enabling functional genomics studies and the genomics-informed breeding pipelines for improving the fruit quality and disease resistance traits.

【 授权许可】

Unknown