Linezolid resistant coagulase negative staphylococci (LRCoNS) with novel mutations causing blood stream infections (BSI) in India

BMC Infectious Diseases

卷:19

Reetika Dawar¹  Raman Sardana¹  Vasundhra Bhandari²  Rajni Gaind³  Gajanand Mittal³  Vandana Rani³  Shimpi Chopra³  P. K. Verma³ 

[1] Indraprastha Apollo Hospital;

[2] National Institute of Animal Biotechnology-DBT;

[3] Vardhman Mahavir Medical College & Safdarjung Hospital;

关键词: Novel mutation;    Linezolid resistance;    CoNS;    cfr;    S. arlettae;    India;   

DOI  :  10.1186/s12879-019-4368-6

来源: DOAJ

【 摘 要 】

Abstract Background Coagulase-negative Staphylococci (CoNS) have emerged as a major causative agent of blood-stream infections (BSI). Linezolid (LZD) is currently used for treating glycopeptide and methicillin-resistant staphylococci. It is important to understand the resistance mechanism and probable transmission of LZD resistant (LR) CoNS within the hospital. Methods Clinically significant LRCoNS from patients with BSI were characterized using MALDI-TOF and 16S rRNA gene sequence analysis. Antimicrobial susceptibility and MIC of vancomycin and LZD were determined. LZD resistance mechanisms using PCR for the cfr gene and mutation in the V domain of the 23S rRNA gene were studied. Results The MIC of LZD ranged from 8 to 32 μg/ml. LR was observed in three different CoNS species from diverse locations within the hospital. The cfr gene was identified in all the isolates. Sequence analysis of V domain region of 23S rRNA gene confirmed mutation in single copy among 12/15 isolates with novel mutations: G2614 T and C2384T. All infections were nosocomially acquired and LZD resistance was emerging in the absence of prior LZD use. Horizontal spread of resistant isolates and cfr gene among diverse species were the probable mechanisms of transmission. Conclusion The study highlights the novel mutations associated with LRCoNS and the importance of surveillance & transmission pathway within the hospital. It also systematically discusses the published information on LRCoNS.

【 授权许可】

Unknown