An ICT-Based Coumarin Fluorescent Probe for the Detection of Hydrazine and Its Application in Environmental Water Samples and Organisms

Frontiers in Bioengineering and Biotechnology

卷:10

Yi Wang¹  Fugang Fan¹  Panpan Chen¹  Xina Liu¹  Chenyang Xu¹  Dongyang Li²  Meiqing Zhu³ 

[1] Anhui Provincial Key Laboratory of Quality and Safety of Agricultural Products, College of Resources and Environment, Anhui Agricultural University, Hefei, China;

[2] Laboratory of Agricultural Information Intelligent Sensing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China;

[3] School of Chemical and Environmental Engineering, Anhui Polytechnic University, Wuhu, China;

关键词: hydrazine;    detection;    density functional theory;    imaging;    fluorescent probe;   

DOI  :  10.3389/fbioe.2022.937489

来源: DOAJ

【 摘 要 】

As an inorganic small molecule pollutant, the toxicity and potential carcinogenicity of hydrazine (N2H4) are of increasing concern. In this work, A water-soluble fluorescent probe (OCYB) based on the intramolecular charge transfer (ICT) mechanism for the detection of hydrazine was designed and synthesized. Taking the advantage of 4-bromobutyryl as the recognition group, the high selectivity of OCYB to N2H4 was confirmed by steady-state fluorescence spectroscopy. The limit of detection (LOD) was calculated to be 78 nM in the DMSO-HEPES (pH 7.4) system. The detection mechanism was verified by NMR, HRMS and density functional theory (DFT) calculations. In addition, OCYB exhibits strong anti-interference ability and an “Off-On” fluorescence enhancement effect. Importantly, OCYB can be used to effectively monitor the fluorescence distribution of N2H4 in environmental water samples and organisms.

【 授权许可】

Unknown