Understanding the molecular mechanisms underlying graft success in grapevine

BMC Plant Biology

卷:19

C. Santos¹  J. E. Eiras-Dias²  J. Brazão²  M. Assunção³  P. Fevereiro³ 

[1] Genetics and Genomics of Plant Complex Traits (PlantX) Laboratory, Instituto de Tecnologia Química e Biológica António Xavier (Green-it Unit), Universidade Nova de Lisboa;

[2] Instituto Nacional de Investigação Agrária e Veterinária (Biotechnology and Genetic Genetic Resources Unit) INIAV-Dois Portos;

[3] Plant Cell Biotechnology Laboratory, Instituto de Tecnologia Química e Biológica António Xavier (Green-it Unit), Universidade Nova de Lisboa;

关键词: Grapevine;    Grafting;    Graft compatibility;    Molecular mechanism of grafting;    Vascular differentiation;    Transcriptional regulation of grafting;   

DOI  :  10.1186/s12870-019-1967-8

来源: DOAJ

【 摘 要 】

Abstract Background Grafting is an intensive commercial practice required to protect the European grapevine against the Phylloxera pest. Rootstocks resistant to this pest are hybrids of American vine species with different levels of compatibility with European Vitis vinifera varieties. Aiming to understand what drives grafting compatibility in grapevine, a transcriptomic approach was used to search for master regulators of graft success. Two scion/rootstock combinations, with different levels of compatibility, were compared in a nursery-grafting context at two stages, at 21 and 80 days after grafting. Results In the most compatible combination, an earlier and higher expression of genes signaling the metabolic and hormonal pathways as well as a reduced expression of genes of the phenolic metabolism and of the oxidative stress response was observed. At 80 days after grafting a higher expression of transcription factors regulating vascular maintenance, differentiation and proliferation was obtained in the most compatible combination. Moreover, lower expression levels of microRNAs potentially targeting important transcription factors related to plant development was observed in the more compatible combination when compared to the less compatible one. Conclusion In this context, a set of regulators was selected as potential expression markers for early prediction of a compatible grafting.

【 授权许可】

Unknown