Calorie Restriction in Adulthood Reduces Hepatic Disorders Induced by Transient Postnatal Overfeeding in Mice

Nutrients

卷:11

Christian Juvet¹  Eulalia Orozco¹  Hassib Chehade¹  Catherine Yzydorczyk¹  Benazir Siddeek¹  Dolores Mosig¹  Umberto Simeoni¹  Thibaud. Rolle¹  JeanBaptiste Armengaud¹  Anithan Krishnasamy¹  Eve Rigal²  Catherine Vergely²  Na Li² 

[1] DOHaD Laboratory, Woman-Mother-Child Department, Division of Pediatrics, Centre Hospitalier Universitaire Vaudois and University of Lausanne, 1011 Lausanne, Switzerland;

[2] Equipe Physiopathologie et Epidémiologie Cérébro-Cardiovasculaires (PEC2, EA7460), UFR Sciences de Santé, Université de Bourgogne Franche-Comté, 21000 Dijon, France;

关键词: liver;    developmental programming;    stress-induced premature senescence;    oxidative stress;    reversibility;    dohad;   

DOI  :  10.3390/nu11112796

来源: DOAJ

【 摘 要 】

Impaired early nutrition influences the risk of developing metabolic disorders in later life. We observed that transient postnatal overfeeding (OF) in mice induces long-term hepatic alterations, characterized by microsteatosis, fibrosis associated with oxidative stress (OS), and stress-induced premature senescence (SIPS). In this study, we investigated whether such changes can be reversed by moderate calorie restriction (CR). C57BL/6 male mice pups were maintained during lactation in litters adjusted to nine pups in the normal feeding (NF) group and three pups in the transient postnatal OF group. At six months of age, adult mice from the NF and OF groups were randomly assigned to an ad libitum diet or CR (daily energy supply reduced by 20%) for one month. In each group, at the age of seven months, analysis of liver structure, liver markers of OS (superoxide anion, antioxidant defenses), and SIPS (lipofuscin, p53, p21, p16, pRb/Rb, Acp53, sirtuin-1) were performed. CR in the OF group reduced microsteatosis, decreased levels of superoxide anion, and increased protein expression of catalase and superoxide dismutase. Moreover, CR decreased lipofuscin staining, p21, p53, Acp53, and p16 but increased pRb/Rb and sirtuin-1 protein expression. CR did not affect the NF group. These results suggest that CR reduces hepatic disorders induced by OF.

【 授权许可】

Unknown