Phloroglucinol and dieckol isolated from Ecklonia cava suppress impaired diabetic angiogenesis; A study of in-vitro and in-vivo

Biomedicine & Pharmacotherapy

卷:138

BoMi Ryu¹  Jin Hwang²  Yu An Lu²  Jun-Geon Je²  K.H.N. Fernando²  You-Jin Jeon²  Hyo-Geun Lee²  Hye-Won Yang² 

[1] Marine Science Institute, Jeju National University, Jeju 63333, South Korea;

[2] Department of Marine Life Science, Jeju National University, Jeju 63243, South Korea;

关键词: Phloroglucinol;    Dieckol;    Angiogenesis;    Diabetes;    EA.hy926 cells;    Zebrafish;   

DOI  :  

来源: DOAJ

【 摘 要 】

Abnormalities in angiogenesis that are associated with diabetes may contribute to vascular complications and result in disabilities and death. Furthermore, an imbalance in angiogenesis in different tissues, including the retina and kidney, can play a role in the pathogenesis of diabetic microvascular complications. Phlorotannins, such as phloroglucinol (PG) and dieckol (DK), which are found in Ecklonia cava exhibit antioxidant and anti-inflammatory activities that improve endothelial function in hypertension. However, reports on the effects of these compounds on diabetes-induced angiogenesis in vivo and in vitro are scarce. In this study, we assessed the antiangiogenic effects of PG and DK on endothelial cells treated with a high concentration of glucose to mimic angiogenesis. In addition, we sought to determine the effects of these compounds on cell proliferation, cell migration, and capillary formation. In silico docking of PG and DK into VEGFR-2 revealed their potential as therapeutic agents against angiogenesis. Further, both compounds were identified to inhibit the formation of the retinal vessel in transgenic zebrafish (flk:EGFP) embryos under high glucose conditions. These findings suggested that PG and DK derived from E. cava are potential inhibitors of angiogenesis in diabetic vascular complications and could, therefore, be used to develop angiogenic agents.

【 授权许可】

Unknown