Nitrogen Removal Performance and Metabolic Pathways Analysis of a Novel Aerobic Denitrifying Halotolerant Pseudomonas balearica Strain RAD-17

Microorganisms

卷:8

Dedong Kong¹  Yu Liu²  Huifeng Lu³  Heping Zhao³  Xiangyang Xu³  Yunjie Ruan⁴  Lei Cai⁵  MohammadJ. Taherzadeh⁶ 

[1] Agricultural Experiment Station, Zhejiang University, Hangzhou 310058, China;

[2] College of Life Science, Zhengjiang University, Hangzhou 310058, China;

[3] Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China;

[4] Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China;

[5] Laboratory of Microbial Resources, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China;

[6] Swedish Centre for Resource Recovery, University of Borås, 50190 Borås, Sweden;

关键词: aerobic denitrification;    pseudomonas balearica rad-17;    nitrogen removal;    metabolic pathways;    bioaugmentation;   

DOI  :  10.3390/microorganisms8010072

来源: DOAJ

【 摘 要 】

An aerobic denitrification strain, Pseudomonas balearica RAD-17, was identified and showed efficient inorganic nitrogen removal ability. The average NO₃⁻-N, NO₂⁻-N, and total ammonium nitrogen (TAN) removal rate (>95% removal efficiency) in a batch test was 6.22 mg/(L∙h), 6.30 mg/(L∙h), and 1.56 mg/(L∙h), respectively. Meanwhile, optimal incubate conditions were obtained through single factor experiments. For nitrogen removal pathways, the transcriptional results proved that respiratory nitrate reductases encoded by napA, which was primarily performed in aerobic denitrification and cell assimilation, were conducted by gluS and gluD genes for ammonium metabolism. In addition, adding the strain RAD-17 into actual wastewater showed obvious higher denitrification performance than in the no inoculum group (84.22% vs. 22.54%), and the maximum cell abundance achieved 28.5 ± 4.5% in a ratio of total cell numbers. Overall, the efficient nitrogen removal performance plus strong environmental fitness makes the strain RAD-17 a potential alternative for RAS (recirculating aquaculture system) effluent treatment.

【 授权许可】

Unknown