In Vitro Fumonisin Biosynthesis and Genetic Structure of Fusarium verticillioides Strains from Five Mediterranean Countries

Microorganisms

卷:8

Francesco Valente¹  Giovanni Beccari¹  Andrea Onofri¹  Lorenzo Covarelli¹  Monika Urbaniak²  Łukasz Stępień²  SallyI. Abd-El Fatah³  VeronicaM. T. Lattanzio⁴  Biancamaria Ciasca⁴ 

[1] Department of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, Italy;

[2] Department of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland;

[3] Food Toxins and Contaminants Department, National Research Centre, Cairo 12622, Egypt;

[4] National Research Council of Italy, Institute of Sciences of Food Production (ISPA-CNR), 70126 Bari, Italy;

关键词: fusarium;    ear rot;    maize;    fumonisins;    fum1;   

DOI  :  10.3390/microorganisms8020241

来源: DOAJ

【 摘 要 】

Investigating the in vitro fumonisin biosynthesis and the genetic structure of Fusarium verticillioides populations can provide important insights into the relationships between strains originating from various world regions. In this study, 90 F. verticillioides strains isolated from maize in five Mediterranean countries (Italy, Spain, Tunisia, Egypt and Iran) were analyzed to investigate their ability to in vitro biosynthesize fumonisin B₁, fumonisin B₂ and fumonisin B₃ and to characterize their genetic profile. In general, 80% of the analyzed strains were able to biosynthesize fumonisins (range 0.03−69.84 μg/g). Populations from Italy, Spain, Tunisia and Iran showed a similar percentage of fumonisin producing strains (>90%); conversely, the Egyptian population showed a lower level of producing strains (46%). Significant differences in fumonisin biosynthesis were detected among strains isolated in the same country and among strains isolated from different countries. A portion of the divergent FUM1 gene and of intergenic regions FUM6-FUM7 and FUM7-FUM8 were sequenced to evaluate strain diversity among populations. A high level of genetic uniformity inside the populations analyzed was detected. Apparently, neither geographical origin nor fumonisin production ability were correlated to the genetic diversity of the strain set. However, four strains from Egypt differed from the remaining strains.

【 授权许可】

Unknown