Detection of UGT1A1*28 Polymorphism Using Fragment Analysis

Chinese Journal of Lung Cancer

卷:20

Weibang GUO¹  Xiaosui HUANG¹  Ying HUANG¹  Hongsui WU¹  Jian SU¹  Zhiyi LV¹  Suqing YANG¹  Xuchao ZHANG¹  Zhi XIE¹  Danxia LU¹ 

[1] Guangdong General Hospital Institute of Lung Cancer, Key Laboratory of Translational Medicine, Medical Research Center, 
Guangdong General Hospital Guangdong Academy of Medical Sciences, Guangzhou 510080, China;

关键词: UGT1A1;    Fragment analysis;    Polymorphism;   

DOI  :  10.3779/j.issn.1009-3419.2017.12.04

来源: DOAJ

【 摘 要 】

Background and objective Uridine-diphosphoglucuronosyl transferase 1A1 (UGT1A1), UGT1A1*28 polymorphism can reduce UGT1A1 enzymatic activity, which may lead to severe toxicities in patients who receive irinotecan. This study tries to build a fragment analysis method to detect UGT1A1*28 polymorphism. Methods A total of 286 blood specimens from the lung cancer patients who were hospitalized in Guangdong General Hospital between April 2014 to May 2015 were detected UGT1A1*28 polymorphism by fragment analysis method. Results Comparing with Sanger sequencing, precision and accuracy of the fragment analysis method were 100%. Of the 286 patients, 236 (82.5% harbored TA6/6 genotype, 48 (16.8%) TA 6/7 genotype and 2 (0.7%) TA7/7 genotype. Conclusion Our data suggest hat the fragment analysis method is robust for detecting UGT1A1*28 polymorphism in clinical practice. It’s simple, time-saving, and easy-to-carry.

【 授权许可】

Unknown