Antimicrobial Effect of 1,25 Dihydroxy Vitamin D on Vibrio cholerae and its Association with Serum 25-Hydroxy Vitamin D Level in Rural Elderly Women: An Experimental Study

Journal of Clinical and Diagnostic Research

卷:15

Joyeta Ghosh¹  Debnath Chaudhuri²  Aditi Nag Chaudhuri³  Indranil Saha⁴ 

[1] Assistant Professor, Department of Dietetics and Nutrition, NSHM Knowledge Campus, Kolkata, West Bengal, India.;

[2] Professor, Department of Biochemistry and Nutrition, All India Institute of Hygiene and Public Health, Kolkata, West Bengal, India.;

[3] Professor, Department of Microbiology, Lady Brabourne College, Kolkata, West Bengal, India.;

[4] Scientist E, ICMR-Centre on Non-communicable Diseases, Kolkata, West Bengal, India.;

关键词: inducible nitric oxide synthase;    macrophages;    superoxide dismutase;    supplementation;   

DOI  :  10.7860/JCDR/2021/49439.15571

来源: DOAJ

【 摘 要 】

Introduction: Aging is associated with a variety of functional,demographic and immunologic changes, responsible forincreasing the incidence and severity of infectious diseases inthe elderly.Aim: To examine the association of serum 25-hydroxy vitaminD status with antimicrobial activity of cultured macrophagesisolated within an exclusively elderly population cohort andalso to examine the effect of in-vitro 1,25 hydroxy vitamin Dsupplementation in it.Materials and Methods: This experimental study wasconducted among 113 elderly women aged between 60 to 70years of age, during the period of April 2014 to August 2018,at Amdanga block, North 24th Parganas, West Bengal, India.Their vitamin D status was assessed by the estimation ofserum 25-hydroxy vitamin D and classified into three groups,sufficient group (60 subjects), insufficient group (27 subjects)and deficient group (26 subjects). Later their Peripheral BloodMononuclear Cells (PBMC) were isolated and cultured fromfresh blood of each and every study subject. Supplementationof 1,25 dihydroxy vitamin D {1,25(OH)2D} was given selectivelyat a dose of 10×10-8 M for 72 hours in the culture media andwere exposed to Vibrio cholerae and inducible nitric oxidesynthase (iNOS), Superoxide Dismutase (SOD) activity andBactericidal activity were screened. Statistical Package for theSocial Sciences (SPSS) software, version 20.0 was used toperform statistical analysis.Results: The sufficient group iNOS activity had significantlyhigher than the other two groups. Whereas in-vitro 1, 25(OH)2Dsupplementation increases iNOS activity significantly among allthree groups. As per the Colony Forming Unit (CFU) reductionrate against Vibrio cholerae infection the sufficient serum 25(OH)D consisting group had significantly (p-value=0.03) high CFUreduction rate than the other two groups. Although in-vitro 1,25(OH)2D supplementation had no significant impact on CFUreduction rate among all three groups.Conclusion: Bacteria killing capacity of macrophages variessignificantly with serum 25-hydroxy vitamin D level of thetarget population. Sufficient group macrophages alwayshad better profile than other two groups. In-vitro 1, 25(OH)2Dsupplementation increases iNOS and SOD activity significantly

【 授权许可】

Unknown