Predicting the Effect of Chemical Factors on the pH of Crystallization Trials

iScience

卷:23

Marko Ristic¹  Janet Newman²  Jobie Kirkwood³  David Hargreaves⁴  Julie Wilson⁵ 

[1] Corresponding author;

[2] AstraZeneca, Darwin Building, Cambridge Science Park, Cambridge, UK;

[3] Collaborative Crystallisation Centre, CSIRO, Parkville, VIC, Australia;

[4] Department of Chemistry, University of York, York, UK;

[5] Department of Mathematics, University of York, York, UK;

关键词: Chemistry;    Structural Biology;    Crystallography;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: In macromolecular crystallization, success is often dependent on the pH of the experiment. However, little is known about the pH of reagents used, and it is generally assumed that the pH of the experiment will closely match that of any buffering chemical in the solution. We use a large dataset of experimentally measured solution pH values to show that this assumption can be very wrong and generate a model that can be used to successfully predict the overall solution pH of a crystallization experiment. Furthermore, we investigate the time dependence of the pH of some polyethylene glycol polymers widely used in protein crystallization under different storage conditions.

【 授权许可】

Unknown