Pretreatment of Dioscorea zingiberensis for Microbial Transformation

International Journal Bioautomation

卷:18

Yuling Zhu¹  Tianxiang Zheng² 

[1] ;

[2] College of Life Science, Shaoxing University, 508 West Ring Road, Yuecheng District, Shaoxing, Zhejiang, P. R. China;

关键词: Microbial transformation;    Pretreatment;    α-rhamnase;    β-glucosidase;   

DOI  :  

来源: DOAJ

【 摘 要 】

The influences of five pretreatments on fungal growth and enzyme production during microbial transformation of Dioscorea zingiberensis (DZW) were studied. The biomass, α-rhamnase and β-glucosidase activities in the fermentation system were employed in the study to determine how each method affected the efficiency of microbial transformation. The fungal strain grew better on the substrate which contained easily utilized carbon source. While lack of carbon source induced the strain produce more glucosidase. Among five pretreatment methods, complex enzymatic hydrolyzation can remove 84.3% starch and 76.5% fibre from DZW in form of sugar, which resulted in high α-rhamnase activity of 2.89 IU/mL and β-glucosidase activity of 8.17 IU/mL in fermentation broth.

【 授权许可】

Unknown