Assessment of Single Cell RNA-Seq Normalization Methods

G3: Genes, Genomes, Genetics

卷:7

关键词: normalization;    scRNA;    statistical index;   

DOI  :  10.1534/g3.117.040683

来源: DOAJ

【 摘 要 】

We have assessed the performance of seven normalization methods for single cell RNA-seq using data generated from dilution of RNA samples. Our analyses showed that methods considering spike-in External RNA Control Consortium (ERCC) RNA molecules significantly outperformed those not considering ERCCs. This work provides a guidance of selecting normalization methods to remove technical noise in single cell RNA-seq data.

【 授权许可】

Unknown