Complete Topological Mapping of a Cellular Protein Interactome Reveals Bow-Tie Motifs as Ubiquitous Connectors of Protein Complexes

Cell Reports

卷:31

Jessica X. Hjaltelin¹  Søren Brunak²  William W. Agace³  Cristina Gomez-Casado³  Kirstine G. Belling³  Kristoffer Niss⁴  Thorsten Joeris⁴ 

[1] Institute of Applied Molecular Medicine, Faculty of Medicine, San Pablo CEU University, 28925 Madrid, Spain;

[2] Mucosal Immunology Group, Department of Health Technology, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark;

[3] Immunology Section, Lund University, BMC D14, 221-84 Lund, Sweden;

[4] Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark;

关键词: biological networks;    bow-tie;    knot protein;    network topology;    network motifs;    functional organization;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The network topology of a protein interactome is shaped by the function of each protein, making it a resource of functional knowledge in tissues and in single cells. Today, this resource is underused, as complete network topology characterization has proved difficult for large protein interactomes. We apply a matrix visualization and decoding approach to a physical protein interactome of a dendritic cell, thereby characterizing its topology with no prior assumptions of structure. We discover 294 proteins, each forming topological motifs called “bow-ties” that tie together the majority of observed protein complexes. The central proteins of these bow-ties have unique network properties, display multifunctional capabilities, are enriched for essential proteins, and are widely expressed in other cells and tissues. Collectively, the bow-tie motifs are a pervasive and previously unnoted topological trend in cellular interactomes. As such, these results provide fundamental knowledge on how intracellular protein connectivity is organized and operates.

【 授权许可】

Unknown