A Novelty System for Biotization of Plant Microshoots and Collection of Natural Compounds

Methods and Protocols

卷:2

MariaAmely Zavattieri¹  Carla Ragonezi¹  PauloGuilherme Leandro de Oliveira²  MárioRui da Costa Basílio e Castro³ 

[1] Banco de GermoplasmaISOPlexis, Campus da Penteada, Universidade da Madeira, 9020-105 Funchal, Portugal;

[2] Departamento de Biologia, Pólo da Mitra Apartado 94, 7002-554 Évora, Portugal;

[3] Institute of Mediterranean Agricultural and Environmental Sciences (ICAAM). Colégio da Mitra, Ap. 94, 7002-554 Évora, Portugal;

关键词: Keywords: biotechnology;    biotization;    chemical analysis;    double-phase medium;    phenolic compounds;    liquid chromatography—diode array detector—mass spectrometry;   

DOI  :  10.3390/mps2010005

来源: DOAJ

【 摘 要 】

An in vitro plant microshoot culture system composed of two phases; a liquid phase overlaid by a floating solid phase, which is described in detail herein. This system is designed to enable the extraction of natural compounds released/disseminated into the liquid phase during root growth, thus facilitating their processing and biochemical characterization. The solid phase holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its penetration into the liquid phase, where the roots are submerged. Both phases can be independently formulated as required for growth optimization of both organisms. Considering the closed system and known variables described in this patent, applications of the described method include testing with pesticides, herbicides, and other similar products.

【 授权许可】

Unknown