Clinical performance and potential of a SARS-CoV-2 detection kit without RNA purification steps

Journal of Laboratory Medicine

卷:45

Fujiya Yoshihiro¹  Kobayashi Ryo²  Moriai Mikako²  Asanuma Koichi²  Kitayama Ikumi²  Yakuwa Yuki²  Endoh Akemi²  Saeki Masachika²  Takahashi Satoshi²  Kondo Takashi²  Katayama Yuki²  Sato Yuki²  Nirasawa Shinya² 

[1] Division of Infection Control, Sapporo Medical University Hospital, Sapporo, Japan;

[2] Division of Laboratory Medicine, Sapporo Medical University Hospital, Sapporo, Japan;

关键词: assay;    covid-19;    detection;    real-time rt-pcr;    sars-cov-2;   

DOI  :  10.1515/labmed-2021-0073

来源: DOAJ

【 摘 要 】

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is rapidly spreading globally. Early diagnosis plays an essential role in controlling the infection. Therefore, early and accurate SARS-CoV-2 detection assays along with easy operation are required. The aim of this study was to compare the clinical performance of the Ampdirect™ 2019-nCoV Detection Kit (SHIMADZU assay), which does not require RNA purification steps, with that of the preexisting SARS-CoV-2 detection assays, which use a purified RNA template.

【 授权许可】

Unknown