Ethylene-regulated immature fruit abscission is associated with higher expression of CoACO genes in Camellia oleifera

Royal Society Open Science

卷:8

Muhammad Sajjad¹  Hongbo Li²  Xiao Hu²  Xiaoling Ma²  Deyi Yuan²  Mi Yang²  Jian Zhang³  Shoufu Gong³ 

[1] Department of Biosciences, COMSATS University Islamabad (CUI), Park Road, Islamabad 45550, Pakistan;

[2] Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education and the Key Laboratory of Non-Wood Forest Products of Forestry Ministry, Central South University of Forestry and Technology, Changsha 410004, People's Republic of China;

[3] Xinyang Agriculture and Forestry University, Xinyang 464000, People's Republic of China;

关键词: ethylene;    CoACO genes;    fruit abscission;    Camellia oleifera;   

DOI  :  10.1098/rsos.202340

来源: DOAJ

【 摘 要 】

Immature fruit abscission is a key limiting factor in Camellia oleifera Abel. (C. oleifera) yield. Ethylene is considered to be an important phytohormone in regulating fruit abscission. However, the molecular mechanism of ethylene in regulating fruit abscission in C. oleifera has not yet been studied. Here, we found that the 1-aminocyclopropane-1-carboxylic acid (ACC) content was significantly increased in the abscission zones (AZs) of abnormal fruits (AF) which were about to abscise when compared with normal fruits (NF) in C. oleifera ‘Huashuo’. Furthermore, exogenous ethephon treatment stimulated fruit abscission. The cumulative rates of fruit abscission in ethephon-treated fruits (ETH-F) on the 4th (35.0%), 8th (48.7%) and 16th (57.7%) days after treatment (DAT) were significantly higher than the control. The ACC content and 1-aminocyclopropane-1-carboxylate oxidase (ACO) activity in AZs of ETH-F were also significantly increased when compared with NF on the 4th and 8th DAT. CoACO1 and CoACO2 were isolated in C. oleifera for the first time. The expressions of CoACO1 and CoACO2 were considerably upregulated in AZs of AF and ETH-F. This study suggested that ethylene played an important role in immature fruit abscission of C. oleifera and the two CoACOs were the critical genes involved in ethylene's regulatory role.

【 授权许可】

Unknown