Cloning and characterization of the lignin biosynthesis genes NcCSE and NcHCT from Neolamarckia cadamba

AMB Express

卷:9

Juncheng Li¹  Ruiqi Pian¹  Xiaoyang Chen¹  Xiaoling Huang¹  Kunxi Ouyang¹  Hao Huang²  Chi D. Nguyen³  Heqiang Huo³  Huaqiang Li⁴ 

[1] Guangdong Key Laboratory for Innovative Development and Utilization of ForestPlant Germplasm, South China Agricultural University;

[2] Guangxi Botanical Garden of Medical Plants;

[3] Mid-Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida;

[4] State_run Leizhou Forestry Bureau;

关键词: Lignin;    NcCSE;    NcHCT;    Neolamarckia cadamba;    DEGs;   

DOI  :  10.1186/s13568-019-0860-z

来源: DOAJ

【 摘 要 】

Abstract Neolamarckia cadamba is an important fast growing tree species used for pulping and wood material in industry for it’s desirable wood properties. As one of the most important content in wood, lignin provides structural integrity, strength, and hydrophobicity to the thickened cell walls and is the major factor contributing to biomass recalcitrance. It does not reduce the palatability of forage grass for animals, but it hinders the isolation of cellulose fibers and the efficient enzymatic depolymerization of cellulose and hemicellulose into fermentable sugars in biorefining processes by limiting the access by hydrolytic enzymes to their polysaccharide substrates. This work focused on analyzing the functions of NcCSE (Caffeoyl Shikimate Esterase, GenBank accession number: MG739672) and NcHCT (Hydroxycinnamoyl Transferase,GenBank accession number: MG739673) in the lignin biosynthetic process in order to improve the potential for utilization of leaves and wood from N. cadamba. The mutant phenotype of cse-2 was dramatically complemented to WT in the stable transgenic lines cse-35S::NcCSE, but overexpression of NcHCT in the cse-2 mutant did not have the same result as cse-35S::NcCSE, providing only partial complementation.

【 授权许可】

Unknown