RNA-Seq of Cyst Nematode Infestation of Potato (Solanum tuberosum L.): A Comparative Transcriptome Analysis of Resistant and Susceptible Cultivars

Plants

卷:11

Sundararaj Nivitha¹  Purushothaman Natarajan²  Umesh K. Reddy²  Mahesh Mahajan³  Palanisamy Sundararaj⁴  Saranya Chandrasekar⁴  Venkatasalam E. Palanisamy⁵  Priyank Hanuman Mhatre⁵ 

[1] College of Science, Northeastern University, Boston, MA 02115, USA;

[2] Department of Biology, Gus R. Douglass Institute, West Virginia State University, Charleston, WV 25112, USA;

[3] Department of Biotechnology, Vilasrao Deshmukh College of Agricultural Biotechnology, Latur 413512, Maharashtra, India;

[4] Department of Zoology, Bharathiar University, Coimbatore 641046, Tamil Nadu, India;

[5] ICAR-Central Potato Research Institute, Regional Station, Udhagamandalam 643004, Tamil Nadu, India;

关键词: RNA-seq;    potato;    cyst nematode;    differentially expressed genes;    disease resistance;   

DOI  :  10.3390/plants11081008

来源: DOAJ

【 摘 要 】

Potato (Solanum tuberosum L.) is an important food crop worldwide, and potato cyst nematodes (PCNs) are among the most serious pests. The identification of disease resistance genes and molecular markers for PCN infestation can aid in crop improvement research programs against PCN infestation. In the present study, we used high-throughput RNA sequencing to investigate the comprehensive resistance mechanisms induced by PCN infestation in the resistant cultivar Kufri Swarna and the susceptible cultivar Kufri Jyoti. PCN infestation induced 791 differentially expressed genes in resistant cultivar Kufri Swarna, comprising 438 upregulated and 353 downregulated genes. In susceptible cultivar Kufri Jyoti, 2225 differentially expressed genes were induced, comprising 1247 upregulated and 978 downregulated genes. We identified several disease resistance genes (KIN) and transcription factors (WRKY, HMG, and MYB) that were upregulated in resistant Kufri Swarna. The differentially expressed genes from several enriched KEGG pathways, including MAPK signaling, contributed to the disease resistance in Kufri Swarna. Functional network analysis showed that several cell wall biogenesis genes were induced in Kufri Swarna in response to infestation. This is the first study to identify underlying resistance mechanisms against PCN and host interaction in Indian potato varieties.

【 授权许可】

Unknown