The Blockade of Store-Operated Calcium Channels Improves Decompression Sickness in Rats

Frontiers in Physiology

卷:10

Wen-I Liao¹  Shi-Jye Chu²  Shih-En Tang⁴  Kun-Lun Huang⁴  Hsin-Ping Pao⁴  Shu-Yu Wu⁴ 

[1] Department of Emergency Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan;

[2] Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan;

[3] Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan;

[4] Graduate Institute of Aerospace and Undersea Medicine, National Defense Medical Center, Taipei, Taiwan;

关键词: store-operated calcium channels;    decompression sickness;    lung injury;    air embolism;    BTP2;   

DOI  :  10.3389/fphys.2019.01616

来源: DOAJ

【 摘 要 】

BackgroundPrevious investigations reveal that BTP2, a store-operated calcium channel blocker, has protective and anti-inflammatory properties in multiple inflammatory diseases. This study investigates whether BTP2 can protect against decompression sickness (DCS) in a rat model.MethodsBTP2 (2 mg/kg) was administered to male Sprague–Dawley rats 30 min before subjecting them to hyperbaric pressure. Control rats were not treated. After decompression, signs of DCS were examined, and samples of bronchoalveolar lavage fluid and lung tissue were obtained for evaluation.ResultsThe incidence and mortality of DCS were decreased significantly in rats treated with BTP2 compared to those treated with dimethyl sulfoxide. BTP2 significantly attenuated DCS-induced lung edema, histological evidence of lung inflammation, necroptosis, and apoptosis, while it decreased levels of tumor necrosis factor alpha, interleukin-6, and cytokine-induced neutrophil chemoattractant-1 in bronchoalveolar lavage fluid. In addition, BTP2 reduced the expression of nuclear factor of activated T cells and early growth response protein 3 in lung tissue. BTP2 also significantly increased the levels of inhibitor kappa B alpha and suppressed the levels of nuclear factor kappa B in lung tissue.ConclusionThe results suggest that BTP2 may has potential as a prophylactic therapy to attenuate DCS-induced injury.

【 授权许可】

Unknown